Extracting Homemade Hyaluronic Acid | Extrayendo ácido hialurónico casero

By Remberto Hurtado, Leonardo Putaré, El Deber:

From Egg to Laboratory: Students Succeed in Extracting Homemade Hyaluronic Acid

Ácido hialurónico con sello universitario.

Hyaluronic acid with a university seal.

Biochemistry students found a way to obtain hyaluronic acid from the eggshell membrane. Their proposal, with medical, cosmetic, and sustainable applications, began as a homemade practice.

A common household waste became a potential accessible and sustainable solution for regenerative medicine, the cosmetics industry, and pharmaceuticals. Five Bolivian biochemistry students, currently in their internship phase, managed to extract hyaluronic acid from the membrane of eggshells.

The idea sprouted from Erika Chura’s family experience. “Since I was little, I saw how my family used the egg film to heal wounds. It was homemade, but it worked,” she recalls. That traditional practice became a scientific hypothesis that gave rise to a research project. The university students, trained at the Universidad Privada Franz Tamayo (Unifranz), decided to look beyond waste and see a valuable resource.

With the support of specialized literature, Mariana Mamani explains that the group discovered the unique properties of the membrane. “We suspected its healing capacity could be due to hyaluronic acid, a molecule known for its role in tissue regeneration. Then everything made sense,” she says.

The discovery was strengthened when they found a local study that used eggshell membranes on mouse wounds. “The treated animals healed faster and without visible scars,” highlights Guadalupe Mery Tarqui. “It was impactful because it scientifically validated what we had learned from our grandmothers.”

The team then set an ambitious goal: to confirm the presence of hyaluronic acid in the membrane and develop a product with clinical and commercial potential. “Hyaluronic acid is very expensive. Creams, eye drops, or injections containing it are usually imported. If we could extract it from a waste product like eggshells, we’d have an affordable and eco-friendly alternative,” argues Tarqui.

It wasn’t easy. The lack of specialized equipment forced them to look for alternatives. “We would have loved to use high-performance liquid chromatography (HPLC), but we don’t have that equipment. We used spectrophotometry and, as a reference standard, we used commercial eye drops containing hyaluronic acid. The similarities allowed us to deduce that we had, in fact, extracted it,” explains Erika Chura.

They also conducted rigorous microbiological controls to ensure the product wasn’t contaminated. “We’re working with organic material, so it was essential to follow strict protocols,” adds Mamani.

One of the biggest challenges was collecting enough raw material. “You need a large quantity of eggshells to get just a few grams of membrane. We made agreements with bakeries and pastry shops. Then we had to wash, dry, and carefully process the membranes,” says Cinthia Aydee Laura.

Even the drying process was problematic. “At first, we dried them in the sun, but they darkened and lost properties. We had to adapt the method to preserve their active components,” explains Celia Teodora, another team member.

The result was a 2% hyaluronic acid gel, although they haven’t yet confirmed its exact purity. “We don’t want to market it without complete quality controls. But we’ve taken the first step,” Erika affirms. The team hopes the project can evolve with more technical and academic support.

Hyaluronic acid has multiple applications: from cosmetic use in creams and skin treatments to medical therapies for conditions like rosacea, osteoporosis, or osteoarthritis. “It’s also used in injections to regenerate cartilage and improve mobility in people with bone wear. If we produce it locally, we could make it more accessible,” Tarqui points out.

The project also has an ecological dimension. “In Bolivia, there’s no recycling culture. Reusing eggshells could reduce the organic waste burden. Turning trash into a high-value input is a step toward a circular economy,” reflects Chura.

The students emphasize that doing science in Bolivia is possible. “Even if we don’t have the best equipment, we can still do research. All it takes is curiosity, commitment, and effort,” says Mariana. “We’re not in the first world, but that doesn’t limit us.”

Motivation also came from faculty support. Dr. Cervando Gutiérrez Foronda guided them through the process and celebrated their determination. The message the researchers leave for future generations is clear: “Don’t get discouraged if you don’t find immediate answers. Research is like searching in the dark. But there’s always a light if you move forward with determination. Many times, the answer lies in the most ordinary things, like a simple eggshell,” she concluded.

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